Second Section: A single domain antibody detects and neutralises toxic Aβ42 oligomers in the Alzheimer’s disease CSF
- Liliana Napolitano
- © 2026 Author(s) |
- CC BY 4.0
- DOI: 10.36253/979-12-215-0993-9.05
This section focuses on the application of the single-domain antibody DesAb-O as a tool for detecting and neutralizing toxic Aβ42 oligomers. Through in vitro ELISA and dot-blot assays, the study demonstrates that DesAb-O selectively detects synthetic Aβ42 oligomers and recognizes them within the complex matrix of cerebrospinal fluid (CSF) from Alzheimer’s Disease patients. Utilizing STED microscopy, the study further characterizes the antibody’s ability to identify oligomers bound to neuronal membranes and internalized into the cytosol. Crucially, the chapter provides evidence that DesAb-O inhibits the interaction between toxic aggregates and the SH-SY5Y cells, thereby preventing mitochondrial dysfunction, membrane destabilization, and neuronal impairment induced by both synthetic species and patient-derived CSF.
- Keywords:
- Alzheimer’s Disease,
- Aβ42 Oligomers,
- Single-domain Antibody,
- ELISA Assay,
- STED Microscopy,
- Neuronal Dysfunction,
- ORCID: 0009-0004-3087-286X
Informazioni sul capitolo
Titolo del capitolo
Second Section: A single domain antibody detects and neutralises toxic Aβ42 oligomers in the Alzheimer’s disease CSF
Autori
Liliana Napolitano
Lingua
Inglese
DOI
10.36253/979-12-215-0993-9.05
Opera sottoposta a peer review
Anno di pubblicazione
2026
Copyright
© 2026 Author(s)
Licenza d'uso
Licenza dei metadati
Informazioni bibliografiche
Titolo del libro
A multidisciplinary approach for the early diagnosis of Alzheimer’s disease and potential therapeutic applications
Autori
Liliana Napolitano
Opera sottoposta a peer review
Numero di pagine
184
Anno di pubblicazione
2026
Copyright
© 2026 Author(s)
Licenza d'uso
Licenza dei metadati
Editore
Firenze University Press
DOI
10.36253/979-12-215-0993-9
ISBN Print
979-12-215-0992-2
eISBN (pdf)
979-12-215-0993-9
eISBN (xml)
979-12-215-0994-6
Collana
Premio Tesi di Dottorato Città di Firenze
ISSN della collana
3103-3881
e-ISSN della collana
3103-3989