Material and Methods
- Liliana Napolitano
- © 2026 Author(s) |
- CC BY 4.0
- DOI: 10.36253/979-12-215-0993-9.03
This chapter describes the materials and methods employed across the three main sections of the study. It details patient recruitment and neuropsychological examination, CSF collection, and biochemical assays such as circular dichroism (CD), dynamic light scattering (DLS), and intrinsic tryptophan fluorescence. The methodological framework also encompasses cell culture models, intracellular calcium measurements, MTT reduction assays, and advanced imaging techniques like STED microscopy. Furthermore, it outlines the purification of DesAb-O and Dimeric-DesAb-O constructs, including the characterization of their binding properties through dot blot analysis and Real-Time based ELISA assay. Furthermore, statistical methodologies and ROC curve analyses used for data evaluation are detailed.
- Keywords:
- Experimental Methods,
- CSF Analysis,
- STED Microscopy,
- Cell Culture,
- Biochemical Assays,
- Statistical Analysis,
- ORCID: 0009-0004-3087-286X
Informazioni sul capitolo
Titolo del capitolo
Material and Methods
Autori
Liliana Napolitano
Lingua
Inglese
DOI
10.36253/979-12-215-0993-9.03
Opera sottoposta a peer review
Anno di pubblicazione
2026
Copyright
© 2026 Author(s)
Licenza d'uso
Licenza dei metadati
Informazioni bibliografiche
Titolo del libro
A multidisciplinary approach for the early diagnosis of Alzheimer’s disease and potential therapeutic applications
Autori
Liliana Napolitano
Opera sottoposta a peer review
Numero di pagine
184
Anno di pubblicazione
2026
Copyright
© 2026 Author(s)
Licenza d'uso
Licenza dei metadati
Editore
Firenze University Press
DOI
10.36253/979-12-215-0993-9
ISBN Print
979-12-215-0992-2
eISBN (pdf)
979-12-215-0993-9
eISBN (xml)
979-12-215-0994-6
Collana
Premio Tesi di Dottorato Città di Firenze
ISSN della collana
3103-3881
e-ISSN della collana
3103-3989