Monografia

Using the Patch-Clamp technique to shed light on ion channels structure, function and pharmacology

  • Roberta Gualdani,

Ion channels are membrane proteins that selectively allow ions to flow down their electrochemical gradient across the cellular membrane. They localize in both plasma and intracellular membranes and regulate a variety of functions such as neuronal excitability, heartbeat, muscle contraction and hormones release. Thus, understanding the molecular mechanism of ion channels function and regulation is one of the key goals of modern Biophysics. During my PhD thesis, by combining patch-clamp measurements with site-direct mutagenesis, fluorophore labeling experiments and pharmacological assays, I explored some functional and structural properties of different ion transporters: the Na+/Ca2+ exchanger (NCX); the large conductance Ca2+-voltage activated K+ channel (BK) channel; the human Transient receptor potential, member A1 (TRPA1) channel.

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Acquisto

Roberta Gualdani

University of Florence, Italy - ORCID: 0000-0003-3617-4953

She graduated in Chemistry at Scuola Normale Superiore in 2008, with a thesis carried out at NEST laboratory in Pisa. She subsequently joined the research team of Prof. Maria Rosa Moncelli (Chemistry Department, University of Florence) in 2009 as PhD student and, since 2012, as postdoctoral fellow. Her project is aiming to investigate how ion channels work and how novel drugs modulate them, by combining fluorescence and electrophysiological techniques.
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  • Anno di pubblicazione: 2013
  • Pagine: 64
  • eISBN: 978-88-6655-453-0
  • Licenza d'uso: CC BY-ND 3.0 IT
  • © 2013 Author(s)
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  • Anno di pubblicazione: 2013
  • eISBN: 978-88-9273-470-8
  • Licenza d'uso: CC BY-ND 3.0 IT
  • © 2013 Author(s)
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  • Anno di pubblicazione: 2013
  • Pagine: 64
Informazioni bibliografiche

Titolo del libro

Using the Patch-Clamp technique to shed light on ion channels structure, function and pharmacology

Autori

Roberta Gualdani

Opera sottoposta a peer review

Numero di pagine

64

Anno di pubblicazione

2013

Copyright

© 2013 Author(s)

Licenza d'uso

CC BY-ND 3.0 IT

Licenza dei metadati

CC0 1.0

Editore

Firenze University Press

DOI

10.36253/978-88-6655-453-0

ISBN Print

978-88-6655-452-3

eISBN (pdf)

978-88-6655-453-0

eISBN (xml)

978-88-9273-470-8

Collana

Premio Tesi di Dottorato

ISSN della collana

2612-8039

e-ISSN della collana

2612-8020

832

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